ABSTRACT
Objective To investigate the activation of local renin-angiotensin system induced by TLR4 signaling pathway in the adipose tissues in diet-induced obesity(DIO)mice. Methods C57BL/6 mice,six-week-old,male,were divided randomly into normal-diet group and high-fat diet group. High-fat diet group was fed for 14 weeks. Mice,which weighted up 20%,were divided into 3 groups:obesity-group(OB,n=5),fenofibrate group (FF,n = 5)and TAK242 group(TAK,n = 5). The fenofibrate group was given fenofibrate(100 mg/kg/day)by oral gavage for a further 2 weeks and the TAK242 group TAK-242(3 mg/kg/d)by intraperitoneal injection for 2 weeks. The 23-week-aged mice were sacrificed and the triglyceride(TG),nonesterified fatty acid(NEFA),glutamic-pyruvic transaminase(ALT),aspartate aminotransferase(AST)were measured. NEFA in liver tissues were measured with ELISA and the expression levels of AT1R,AGT and TLR4 were measured by RT-PCR. Results TAK-242(Resatorvid)inhibited the expressions of AT1R,AGT and TLR4 in adipose tissues. The effects of fenofi-brate were different in adipose tissues and liver tissues. Conclusion Activation of TLR4 signaling path way ac-tives the local RAS in adipose tissues. The expressions of AT1R and AGT in adipose tissue are significantly inhibit-ed by TAK-242.